The Potential of Robusta Coffee Bean (Coffea Canephora) Extract in Vivo Healing of Incision Wounds in Sprague Dawley Rats

Cuts are a common type of wound and require proper treatment to accelerate healing and prevent infection. One plant that has potential for wound healing is robusta coffee (Coffea canephora). This study aims to evaluate the effectiveness of thick extract of robusta coffee beans on wound healing in Sprague Dawley white rats. The study used a laboratory experimental method with a Post-Test Only Control Group Design. A total of 25 rats were divided into five groups, namely negative control (no treatment), positive control (povidone iodine), and three treatment groups of robusta coffee extract with concentrations of 10%, 20%, and 30%. Treatment was given once a day for 14 days, and histopathological examination was performed on the 15th day. Macroscopic observation data used Nagaoka scoring, then all macroscopic and histopathological data were analyzed with SPSS using the Kruskal-Wallis and Mann-Whitney tests. The results showed that all concentrations of robusta coffee bean extract improved wound healing and tissue regeneration compared to negative and positive controls, with significant differences between groups (p < 0.05). Concentrations of 20% and 30% robusta coffee bean extract provided the most optimal effect in accelerating healing and tissue regeneration.